What product of transcription serves as the code for synthesizing new proteins through the process of translation?

BIO205, Microbiology Quiz 4

Question

1 of 30

What product of transcription serves as the code for synthesizing new proteins through the process of translation?

A new strand of DNA

rRNA

tRNA

mRNA

None of the above

2 of 30

An enzyme produced in response to the presence of a substrate is called ________.

an inducible enzyme

a repressible enzyme

a restriction enzyme

an operator

a promoter

3 of 30

Transformation is the transfer of DNA from a donor to a recipient cell ________.

by a bacteriophage

as naked DNA in solution

by cell-to-cell contact

by crossing over

by sexual reproduction

4 of 30

Genetic change in bacteria can be brought about by ________.

mutation

conjugation transduction

transformation

All of the above

5 of 30

Which of the following is not true of a bacterium that is R+?

R+ refers to the possession of a plasmid.

R+ can be transferred to a recipient cell.

It is resistant to certain drugs and heavy metals.

It is F+.

None of the above

6 of 30

According to the operon model, for the synthesis of an inducible enzyme to occur, the ________.

end-product must not be in excess

substrate must bind to the enzyme

substrate must bind to the repressor

repressor must bind to the operator

repressor must not be synthesized

7 of 30

Synthesis of a repressible enzyme is stopped by ________.

the allosteric transition

the substrate binding to the repressor

the corepressor binding to the operator

the corepressor-repressor binding to the operator

the end-product binding to the promoter Figure 8.2

8 of 30

In Figure 8.2, if base 4 is thymine, what is base 4′?

Adenine

Thymine

Cytosine

Guanine

Uracil

9 of 30

In Figure 8.2, if base 4 is thymine, what is base 11′?

Adenine

Thymine

Cytosine

Guanine

Uracil

10 of 30

In Figure 8.2 shows a figure of an unwound segment of DNA being replicated, base 2 is attached to _________.

ribose

phosphate

deoxyribose

thymine

Can’t tell

11 of 30

The reaction catalyzed by reverse transcriptase is ________.

DNA mRNA

mRNA cDNA

mRNA protein

DNA DNA

None

12 of 30

Subunit vaccines can be made by genetic engineering of yeast cells. A side effect of this kind of vaccination might be ________.

the disease

a yeast infection

due to extraneous material

that the vaccine doesn’t provide immunity

None of the above

13 of 30

E. coli can make insulin because ________.

it needs to regulate its cell-glucose level

it’s an ancient gene that now has no function

the insulin gene was inserted into it

it picked up the insulin gene from another cell

No reason; it doesn’t make insulin.

14 of 30

The value of cDNA in genetic engineering is that ________.

it lacks exons

it lacks introns

it’s really RNA

None of the above

15 of 30

Which enzyme does not make sticky ends?

Enzyme Recognition

BamHI G¯GATCC

CCTAG­G

Enzyme Recognition

EcoRI G¯AATTC

CTTAA­G

Enzyme Recognition

HaeIII GG¯CC

CC­GG

Enzyme Recognition

HindIII A¯AGCTT

TTCGA­A

Enzyme Recognition PstI CTGC¯G

G­ACGTC

16 of 30

Which enzyme would cut this strand of DNA:

GCATGGATCCCAATGC?

Enzyme Recognition

BamHI G¯GATCC

CCCTAG­G

Enzyme Recognition

EcoRI G¯AATTC

CTTAA­G

Enzyme Recognition

HaeIII GG¯CC

CC­GG

Enzyme Recognition

HindIII A¯AGCTT

TTCGA­A

Enzyme Recognition

PstI CTGC¯G

G­ACGTC

17 of 30

An example of gene therapy is ________.

insertion of the insulin gene in E. coli.

insertion of the insulin gene in a mammalian cell culture

insertion of the insulin gene in a diabetic person’s pancreas cells

injection of insulin into a diabetic person

None of the above 18 of 30

Humans use __________ to select desirable breeds of animals or strains of plants to cultivate.

replica plating

mutation

artificial selection

natural selection

All of the above 19 of 30

A restriction fragment is ________.

a gene

a segment of DNA

a segment of RNA

None of the above

20 of 30

Which of the following can be used to make recombinant DNA?

protoplast fusion

Tungsten "bullets"

microinjection

Transformation

All of the above

21 of 30

What do you typically call segments of DNA?

Chromosome

Genome

Genes

Fragments

22 of 30

When DNA becomes mRNA what has happened?

An organism has died

Translation

Transcription

Ligation

23 of 30

What is the purpose of the Ames test?

To test for AIDS

To screen mutagens suspected of causing cancer in humans

To diagnose cancer

To see if DNA has been destroyed

24 of 30

What is typically the final step in genetic engineering?

Plasmid is formed

Vector is selected

DNA is copied

Genes or proteins are harvested

25 of 30

How many properties of vectors are there?

3

2

1

4

26 of 30

What do you call the science of determining what all of the proteins are expressed in a cell?

Genomics

Southern blotting

Proteomics

Orthocloning

27 of 30

Which of the following groups of microorganisms has the rRNA loop present

Archaea

Eukarya

Bacteria

Viruses

28 of 30

Which of the following is a Class?

Eukarya

Proteobacteria

Carnivore

Mammalia

29 of 30

Why are the use of gram staining and acid fast staining used in a clinical setting?

They are expensive

They are slow

They are quick and provide a good starting point for testing and information

They conclusively identify your organism

30 of 30

What technique is used to identify bacteria in a sample without culturing the bacteria?

Fatty acid profiling

Flow Cytometry

Phage Typing

Serology

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